ABSTRACT
Decreasing of harpy eagle (Harpia harpyja) populations in natural environments, mainly in non-preserved areas, makes captive population management an important contribution to genetic diversity conservation. The aim of this study is to evaluate hematological parameters for captive harpy eagles maintained at the wild animals breeding center of Itaipu Binacional, Paraná State, Brazil. Fourteen blood samples from nine harpy eagles were collected from animals of both sexes, of different ages and with no clinical signs of disease. Significant variations were found in haematological values of hematocrit, hemoglobin, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukocyte, a relative number of heterophils, absolute and relative number of lymphocytes, monocytes, eosinophils, basophils and plasma protein between groups of young (less than six months old) and adult birds. Comparing males and females there was variation in the values of erythrocytes, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) on heterophils, absolute and relative number of lymphocytes, eosinophils and basophils. There was also variation in the values of red blood cells, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukocyte count, absolute number of lymphocytes, eosinophils and basophils among birds that study compared to another reference birds. Due to the limited information available on harpy eagle hematology, this study will be useful to the clinical assessment of birds maintained in captivity...
A diminuição de populações de harpia (Harpia harpyja) em ambientes naturais, principalmente em áreas não preservadas, torna a gestão população cativa uma contribuição importante para a conservação da diversidade genética. O objetivo deste estudo é avaliar os parâmetros hematológicos de harpias em cativeiro mantidas para os animais selvagens de reprodução centro da Itaipu Binacional, Paraná, Brasil. Quatorze amostras de sangue de nove harpias foram coletadas de animais de ambos os sexos, de diferentes idades e sem sinais clínicos da doença. Variações significativas foram encontradas nos valores hematológicos de hematócrito, hemoglobina, hemoglobina corpuscular média (HCM), concentração de hemoglobina corpuscular média (CHCM), de leucócitos, um número relativo de heterófilos, absoluta e número relativo de linfócitos, monócitos, eosinófilos, basófilos e proteínas plasmáticas entre grupos de jovens (menos de seis meses de idade) e adultos aves. Comparando homens e mulheres houve variação nos valores de eritrócitos, hemoglobina, volume corpuscular médio (VCM), hemoglobina corpuscular média (HCM) em heterófilos, o número absoluto e relativo de linfócitos, eosinófilos e basófilos. Houve também variação nos valores de células vermelhas do sangue, hematócrito, hemoglobina, volume corpuscular médio (VCM), hemoglobina corpuscular média (HCM), concentração de hemoglobina corpuscular média (CHCM), contagem de leucócitos, número absoluto de linfócitos, eosinófilos e basófilos entre aves que estudam em comparação com outras aves de referência. Devido à pouca informação disponível sobre hematologia harpia, este estudo será útil para a avaliação clínica das aves mantidas em cativeiro...
Subject(s)
Animals , Blood Chemical Analysis/veterinary , Raptors/blood , Natural Reservations/prevention & control , Sex Distribution , Hematologic Tests/veterinary , Animal Welfare , Raptors/anatomy & histology , Housing, AnimalABSTRACT
Basic hematological information about the avian in its natural habitat is limited. In this study we aimed at investigating, in the peripherical blood of the roadside hawk, Buteo magnirostris, the number of thrombocytes and their morphological, cytochemical and ultrastructural aspects. Blood smears were submited to the following methods: Leishman for morphological analysis and specific methods for the characterization of four kinds of macromolecules of different chemical nature: Glycogen, lysosomal enzymes (myeloperoxidase and the acid phosphatase), basic polyaminoacid rich proteins and phospholipids. The average number of thrombocytes was 26.8 x 103 ± 10,2 / µL. Structurally the thrombocytes present predominantly elliptic shape, with dense and clumped nuclear chromatin. The cytoplasm is clear, vacuolated and may contain one or two purple azurophilic granules at the poles. The thrombocytes presented positive cytochemical reaction to glycogen, polyaminoacid and for acid phosphatase. Ultrastructurally, the thrombocytes appear as elliptical or spherical cells containing Golgi, much smooth endoplasmic reticulum, small mitochondria, dense osmiophilic granules, microtubules at the outermost zone of the cell and large vacuoles connected to the canalicular system or opened to the surface.